Goldenseal (Hydrastis canadensis)
Human MDR1 and MRP1 Recognize Berberine as Their Transport Substrate CSV JSON

To examine whether human ATP-binding cassette (ABC) transporters play a role in the detoxification of plant alkaloid berberine, we investigated berberine transport using multidrug resistance protein1 (MDR1) and multidrug resistance-associated protein1 (MRP1). Cells expressing MDR1 or MRP1 accumulated less berberine. Berberine accumulation depended on the cellular ATP level, and was reversed by typical inhibitors of MDR1, suggesting that human MDR1 and MRP1 directly efflux berberine as their substrate.

PMID: 17213652


1 . Berberine Transport by P-gp (id=NPDI-d4PkwQ)

In Vitro Transporter Kinetics Experiment

Transport Activity was detected.  Cutoff used — not specified

berberine 19012197

  • P-gp (ABCB1)

Cell system Other cells

Human cultured KB3-1 cells


Fig 2 MDR1

Sample Compound measured Value Measurement Study sequence Additional information N replicates

Experimental Conditions

Assay Conditions

90 min

37 deg C

10 μM