- Natural product: Kratom (Mitragyna speciosa)
- Associated study: Evaluation of selected Malaysian medicinal plants on phase I drug metabolizing enzymes, CYP2C9, CYP2D6 and CYP3A4 activities in vitro
In Vitro Enzyme Inhibition Experiment
Inhibition of CYP2D6 by M. speciosa methanolic extract
Inhibition was detected. Cutoff used — Not specified.
- CYP2D6 4173631
Recombinant expression system Baculovirus-insect cells
Cytochrome B5 Not available
Results
% enzyme inhibition versus precipitant concentration:
Values estimated from Figure 1d. In some cases, SEM could not be estimated from the provided figure.
0.01 µg/mL: 25 ± Unknown%
0.1 µg/mL: 21 ± Unknown%
1 µg/mL: 35 ± 1%
10 µg/mL: 78 ± 0%
100 µg/mL: 91 ± 3%
1000 µg/mL: See above data
| Sample | Compound measured | Value | Measurement | Study sequence | Additional information | N replicates |
|---|
Experimental Conditions
M. speciosa methanolic extract was prepared in-house from the leaves of the plant. This assay was carried out using the P450-Glo™ Screening Systems from Promega, USA.
The minus-P450 wells (using luciferin-free water) were negative controls.
Commercially available
100 µL
65 min
MgCl2
NADPH regenerating system
Not available
Not available
0.01-1000 µg/mL