- Compound: berberine
- Associated study: Inhibitory Mechanisms of Human CYPs by Three Alkaloids Isolated from Traditional Chinese Herbs
| Natural product species | Study name |
|---|---|
| Goldenseal (Hydrastis canadensis) | Goldenseal root extract Nuclear Receptor Induction study - Ole Miss |
| Goldenseal (Hydrastis canadensis) | Tetrandrine Potentiates the Hypoglycemic Efficacy of Berberine by Inhibiting P-Glycoprotein Function |
| Goldenseal (Hydrastis canadensis) | Inhibition of P-Glycoprotein by HIV Protease Inhibitors Increases Intracellular Accumulation of Berberine in Murine and Human Macrophages |
| Goldenseal (Hydrastis canadensis) | Identification of novel dietary phytochemicals inhibiting the efflux transporter breast cancer resistance protein (BCRP/ABCG2) |
| Goldenseal (Hydrastis canadensis) | Visit the Studies page to view other studies about Goldenseal (Hydrastis canadensis) |
In Vitro Enzyme Inhibition Experiment
3A4 preincubation without NADPH
Inhibition was detected. Cutoff used — not specifed
- CYP3A4 4306811
Recombinant expression system Baculovirus-insect cells
Cytochrome B5 Co-expressed
Results
Results estimated above from 30 min of preincubation (Fig 2)
| Sample | Compound measured | Value | Measurement | Study sequence | Additional information | N replicates |
|---|
Experimental Conditions
141-pmol and 149-pmol
Commercially available
82636 and 38275
400 μL
10 min
NADPH
NADPH regenerating system
P450 reductase
100 μM
0,5,15,30, and 45 min
No NADPH with precipitant
50.5 μM