3A4 preincubation without NADPH CSV JSON

In Vitro Enzyme Inhibition Experiment

3A4 preincubation without NADPH

Inhibition was detected.  Cutoff used — not specifed

testosterone

Berberine 19012197

6beta-Hydroxytestosterone

  • CYP3A4 4306811

Recombinant expression system Baculovirus-insect cells
Cytochrome B5 Co-expressed

Results

Results estimated above from 30 min of preincubation (Fig 2)

Compound measured Measurement Value Study sequence Additional information N replicates

Experimental Conditions

141-pmol and 149-pmol

Commercially available

82636 and 38275

400 μL

10 min

NADPH
NADPH regenerating system
P450 reductase

100 μM

0,5,15,30, and 45 min

No NADPH with precipitant

50.5 μM