- Natural product: Kratom (Mitragyna speciosa)
- Associated study: Evaluation of selected Malaysian medicinal plants on phase I drug metabolizing enzymes, CYP2C9, CYP2D6 and CYP3A4 activities in vitro
In Vitro Enzyme Inhibition Experiment
Inhibition of CYP2C9 by M. speciosa methanolic extract
Negligible Inhibition was detected. Cutoff used — Not specified.
- CYP2C9 4309227
Recombinant expression system Baculovirus-insect cells
Cytochrome B5 Not available
Results
IC50 not determined due to the less than 50% of inhibition.
% enzyme inhibition versus precipitant concentration:
Values estimated from Figure 1d. In some cases, SEM could not be estimated from the provided figure.
0.01 µg/mL: 20 ± Unknown%
0.1 µg/mL: 18 ± Unknown%
1 µg/mL: 22 ± 6%
10 µg/mL: 23 ± 6%
100 µg/mL: 26 ± 2%
1000 µg/mL: See above data
| Sample | Compound measured | Value | Measurement | Study sequence | Additional information | N replicates |
|---|
Experimental Conditions
M. speciosa methanolic extract was prepared in-house from the leaves of the plant. This assay was carried out using the P450-Glo™ Screening Systems from Promega, USA.
The minus-P450 wells (using luciferin-free water) were negative controls.
Commercially available
100 µL
50 min
MgCl2
NADPH regenerating system
Not available
Not available
0.01-1000 µg/mL