In Vitro Transporter Inhibition Experiment

Inhibition of P-gp by Mitragynine

Inhibition was detected.  Cutoff used —

positive control verapamil (22.3± 1.4 μM)

calcein-AM

Mitragynine

  • P-gp (ABCB1)

Transfected / siRNA knock-out / injected cells MDCK transfected cells

Results

positive control verapamil (22.3± 1.4 μM)


Fig 1S (with exact values in text pg 574)

Compound measured Measurement Value Study sequence Additional information N replicates

Experimental Conditions

Cell Culture Conditions

70 000 cells/well

200 μL

Transwell

2

30-42

Viability and Function

>1100 ohm/cm2

TEER measurements and Ly permeability.

Assay Conditions

120 min

37 deg C

7.4

5 uM

5 uM

ChromaDex