CBD-Rh123 (Flow-Cytometry Assay) CSV JSON

In Vitro Transporter Inhibition Experiment

CBD-Rh123 (Flow-Cytometry Assay)

Inhibition was detected.  Cutoff used —

Inhibition of Rh123 efflux caused by the treatment of 5 μM PSC833 was defined as 100% inhibition

rhodamine 123

cannabidiol (cbd)

  • P-gp (ABCB1)

Transfected / siRNA knock-out / injected cells LLC-PK1 transfected cells

Results

Accumulation estimated from Fig 3a at 30 μM of CBD. p < 0.01 versus control
%inhibition estimated from Fig 5 at 100 μM.
IC50 found within text

Sample Compound measured Value Measurement Study sequence Additional information N replicates

Experimental Conditions

Cell Culture Conditions

1x10^5 cells/mL/well

Assay Conditions

60 m

37'C

7.4

5 µM

2, 5, 10, 20, 50, 100 µM

Sigma-Aldrich (St. Louis, MO)