3A4 preincubation with NADPH CSV JSON

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3A4 preincubation with NADPH

Object compound

Precipitant

Object metabolite investigated

Enzymes involved in metabolite pathway

Test system used

Recombinant expression system Baculovirus-insect cells
Cytochrome B5 Co-expressed

Results above from 30 min preincubation with NADPH (fig 2)
Table 2

Sample	Compound measured	Value	Measurement	Study sequence	Additional information	N replicates

Protein concentration

141-pmol and 149-pmol

Test system preparation

Commercially available

Test system lot number

82636 and 38275

Incubation volume

400 μL

Incubation time

10 min

Co-factors

NADPH
NADPH regenerating system
P450 reductase

Object concentrations tested

100 μM

Precipitant pre-incubation time

0,5,15,30, and 45 min

Precipitant pre-incubation condition

NADPH with precipitant

Precipitant concentrations tested

50.5 μM

NOTE