2D6 preincubation without NADPH CSV JSON

In Vitro Enzyme Inhibition Experiment

2D6 preincubation without NADPH

Inhibition was detected.  Cutoff used — not specified

testosterone

berberine 19012197

6beta-hydroxytestosterone

  • CYP2D6 4173631

Recombinant expression system Baculovirus-insect cells
Cytochrome B5 No

Results

Estimate from Fig 2 at 30 mins

Sample Compound measured Value Measurement Study sequence Additional information N replicates

Experimental Conditions

100-pmol CYP/mg

Commercially available

73755

400 μL

25 min

NADPH
NADPH regenerating system
P450 reductase

100 μM

0,5,15,30, and 45 min

No NADPH with precipitant

7.43-μM