- Natural product: Goldenseal (Hydrastis canadensis)
- Associated study: Inhibitory Mechanisms of Human CYPs by Three Alkaloids Isolated from Traditional Chinese Herbs
In Vitro Enzyme Inhibition Experiment
2D6 pre-incubation with NADPH
Inhibition was detected. Cutoff used — not specified
- CYP2D6 4173631
Recombinant expression system Baculovirus-insect cells
Cytochrome B5 No
Results
Estimated from fig 2 at 30 mins
Table 2
Sample | Compound measured | Value | Measurement | Study sequence | Additional information | N replicates |
---|
Experimental Conditions
100-pmol CYP/mg
Commercially available
73755
400 μL
25 min
NADPH
NADPH regenerating system
P450 reductase
100 μM
0,5,15,30, and 45 min
NADPH with precipitant
7.43-μM