No inhibition of CYP3A4 by mitragynine CSV JSON

In Vitro Enzyme Inhibition Experiment

No inhibition of CYP3A4 by mitragynine

Negligible Inhibition was detected.  Cutoff used —

High probability of interaction if their IC50 values are less than 1 µM. If IC50 values are between 1 and 10 µM, they have moderate possibility in interaction. Low drug-drug interactions if their IC50 values are greater than 10 µM.



  • CYP3A4 4306811

Recombinant expression system Baculovirus-insect cells
Cytochrome B5 Co-expressed


IC50 values from Table 1, Ki values from Table 3

% enzyme inhibition versus precipitant concentration:
Values estimated from Figure 2. In some cases, SEM could not be estimated from the provided figure.

0.02 μM: -24 ± 0%
0.2 μM: -21 ± 5%
2 μM: -2 ± 1%
20 μM: 25 ± 2%
200 μM: See above data

Sample Compound measured Value Measurement Study sequence Additional information N replicates

Experimental Conditions

Mitragynine was isolated in-house. This assay was carried out using the P450-Glo™ Screening Systems from Promega, USA.

Commercially available

50 min

NADPH regenerating system

Not available


0.02-200 µM