Experiment #NPDI-Cuu5WA CSV JSON

In Vitro Enzyme Inhibition Experiment

Inhibition was detected.  Cutoff used — N/A

midazolam 708298

epigallocatechin gallate 19058494

1'-hydroxymidazolam

  • CYP3A4 4306811

Recombinant expression system Not available
Cytochrome B5 Not available

Results

The apparent Ki value is presented in the table.
Additional study using TA1538 cell membrane fractions of P450 reductase found that (-)-epigallocatechin gallate inhibited this enzyme competitively with an apparent Ki value of 2.5 µM.

Sample Compound measured Measurement Value Study sequence Additional information N replicates

Experimental Conditions

Experiments were conducted using TA1538 cell membrane fractions expressing P450 reductase and individual CYP isoforms.

CYP: 60 pmol/mL

In-house preparation

0.5 mL

5 min

NADPH regenerating system

Not available

Not available

10 µM

10 µM