- Natural product: Green tea (Camellia sinensis)
- Associated study: Inhibition by green tea catechins of metabolic activation of procarcinogens by human cytochrome P450
In Vitro Enzyme Inhibition Experiment
Inhibition was detected. Cutoff used — N/A
- CYP3A4 4306811
Recombinant expression system Not available
Cytochrome B5 Not available
Results
The apparent Ki value is presented in the table.
Additional study using TA1538 cell membrane fractions of P450 reductase found that (-)-epigallocatechin gallate inhibited this enzyme competitively with an apparent Ki value of 2.5 µM.
| Sample | Compound measured | Value | Measurement | Study sequence | Additional information | N replicates |
|---|
Experimental Conditions
Experiments were conducted using TA1538 cell membrane fractions expressing P450 reductase and individual CYP isoforms.
CYP: 60 pmol/mL
In-house preparation
0.5 mL
5 min
NADPH regenerating system
Not available
Not available
10 µM
10 µM