In Vitro Transporter Inhibition Experiment
Inhibition of MATE2-K by Green Tea
Inhibition was detected. Cutoff used — >20% inhibition vs. control
- MATE2-K (SLC47A2)
Transfected / siRNA knock-out / injected cells HEK293 transfected cells
Percent inhibition in MATE2-K cells was estimated from Fig. 1. The IC50 value for inhibition of metformin by green tea (0.1-30% v/v) could not be calculated.
|Compound measured||Measurement||Value||Study sequence||Additional information||N replicates|
Sodium butyrate (10 mM) was added 24 h prior to uptake experiments to nonspecifically induce transporter expression.
5.0 x 10^5 cells/well
12-well poly-D-lysine coated plates
37 deg C
Healthya green tea (Kao Corp., Tokyo, Japan)