Percent inhibition of CYP3A4 by epicatechin gallate CSV JSON

Percent inhibition of CYP3A4 by epicatechin gallate

Object compound

Precipitant

Object metabolite investigated

Enzymes involved in metabolite pathway

Test system used

Recombinant expression system Not available
Cytochrome B5 Not available

Percent inhibition at 100 µM as estimated from Fig. 3 is presented.

Sample	Compound measured	Value	Measurement	Study sequence	Additional information	N replicates

Experiments were conducted using TA1538 cell membrane fractions expressing P450 reductase and individual CYP isoforms.

Protein concentration

CYP: 60 pmol/mL

Test system preparation

In-house preparation

Incubation volume

0.5 mL

Incubation time

5 min

Co-factors

NADPH regenerating system

Protein linearity

Not available

Time linearity

Not available

Object concentrations tested

10 µM

Precipitant concentrations tested

0-100 µM

NOTE