Cannabis (Cannabis sativa)
Characterization of Human Hepatic and Extrahepatic UDP-Glucuronosyltransferase Enzymes Involved in the Metabolism of Classic Cannabinoids
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Tetrahydrocannabinol (Delta(9)-THC), the primary psychoactive ingredient in marijuana, is subject to cytochrome P450 oxidation and subsequent UDP-glucuronosyltransferase (UGT)-dependent glucuronidation. Many studies have shown that CYP2C9 and CYP3A4 are the primary enzymes responsible for these cytochrome P450-dependent oxidations, but little work has been done to characterize phase II metabolic pathways. In this study, we test the hypothesis that there are specific human UGTs responsible for classic cannabinoid metabolism. The activities of 12 human recombinant UGTs toward classic cannabinoids [cannabinol (CBN), cannabidiol (CBD), (-)-Delta(8)-THC, (-)-Delta(9)-THC, (+/-)-11-hydroxy-Delta(9)-THC (THC-OH), and (-)-11-nor-9-carboxy-Delta(9)-THC (THC-COOH)] were evaluated using high-performance liquid chromatography-tandem mass spectrometry and labeling assays. Despite activity by UGT1A1, 1A3, 1A8, 1A9, 1A10, and 2B7 toward CBN, CBD, THC-OH, and THC-COOH, only selected UGTs demonstrate sufficient activity for further characterization of steady-state kinetics. CBN was the most recognized substrate as evidenced by activities from hepatic UGT1A9 and extrahepatic UGT1A7, UGT1A8, and UGT1A10. These results may reflect the introduction of an aromatic ring to Delta(9)-THC, leading to favorable pi stacking with phenylalanines in the UGT active site. Likewise, oxidation of Delta(9)-THC to THC-OH results in UGT1A9 and UGT1A10 activity toward the cannabinoid. Further oxidation to THC-COOH surprisingly leads to a loss in metabolism by UGT1A9 and UGT1A10, while creating a substrate recognized by UGT1A1 and UGT1A3. The resulting glucuronide of THC-COOH is the main metabolite found in urine, and thus these hepatic enzymes play a critical role in the metabolic clearance of cannabinoids. Taken together, glucuronidation of cannabinoids depends on upstream processing including enzymes such as CYP2C9 and CYP3A4.
1 . THC-OH-UGT1A9 (id=NPDI-qk8qvw)
In Vitro Enzyme Kinetics Experiment
Metabolism was detected.
- UGT1A9
Recombinant expression system
Baculovirus-insect cells
Cytochrome B5 No
Results
Table 2
| Sample | Compound measured | Value | Measurement | Study sequence | Additional information | N replicates |
|---|
Experimental Conditions
Metabolite formation study
5 μg
In-house preparation
30 μL
90 min (screening), 30 min (kinetics)
MgCl2
UDPGA
2 . THC-OH-UGT1A10 (id=NPDI-CWIhCg)
In Vitro Enzyme Kinetics Experiment
Metabolism was detected.
- UGT1A10
Recombinant expression system
Baculovirus-insect cells
Cytochrome B5 No
Table 2
Results
Table 2
| Sample | Compound measured | Value | Measurement | Study sequence | Additional information | N replicates |
|---|
Experimental Conditions
Metabolite formation study
5 μg
In-house preparation
30 μL
90 min (screening), 30 min (kinetics)
MgCl2
UDPGA
3 . THC-COOH-UGT1A1 (id=NPDI-GT0H0Q)
In Vitro Enzyme Kinetics Experiment
Metabolism was detected.
- UGT1A1 40782950
Recombinant expression system
Baculovirus-insect cells
Cytochrome B5 No
Results
Table 2
| Sample | Compound measured | Value | Measurement | Study sequence | Additional information | N replicates |
|---|
Experimental Conditions
Metabolite formation study
5 μg
In-house preparation
30 μL
90 min (screening), 30 min (kinetics)
MgCl2
UDPGA
4 . THC-COOH-UGT1A3 (id=NPDI-GNkg_Q)
In Vitro Enzyme Kinetics Experiment
Metabolism was detected.
- UGT1A3
Recombinant expression system
Baculovirus-insect cells
Cytochrome B5 No
Table 2
Results
Table 2
| Sample | Compound measured | Value | Measurement | Study sequence | Additional information | N replicates |
|---|
Experimental Conditions
Metabolite formation study
5 μg
In-house preparation
30 μL
90 min (screening), 30 min (kinetics)
MgCl2
UDPGA
5 . CBN-UGT1A10 (id=NPDI-PxRYvA)
In Vitro Enzyme Kinetics Experiment
Metabolism was detected.
- UGT1A10
Recombinant expression system
Baculovirus-insect cells
Cytochrome B5 No
Table 2
Results
Table 2
| Sample | Compound measured | Value | Measurement | Study sequence | Additional information | N replicates |
|---|
Experimental Conditions
Metabolite formation study
5 μg
In-house preparation
30 μL
90 min (screening), 30 min (kinetics)
MgCl2
UDPGA
6 . CBN-UGT1A9 (id=NPDI-akOrWg)
In Vitro Enzyme Kinetics Experiment
Metabolism was detected.
- UGT1A9
Recombinant expression system
Baculovirus-insect cells
Cytochrome B5 No
Table 2
Results
Table 2
| Sample | Compound measured | Value | Measurement | Study sequence | Additional information | N replicates |
|---|
Experimental Conditions
Metabolite formation study
5 μg
In-house preparation
30 μL
90 min (screening), 30 min (kinetics)
MgCl2
UDPGA
7 . CBN-UGT1A7 (id=NPDI-Qoqt5Q)
In Vitro Enzyme Kinetics Experiment
Metabolism was detected.
- UGT1A7
Recombinant expression system
Baculovirus-insect cells
Cytochrome B5 No
Table 2
Results
Table 2.
| Sample | Compound measured | Value | Measurement | Study sequence | Additional information | N replicates |
|---|
Experimental Conditions
Metabolite formation study
5 μg
In-house preparation
30 μL
90 min (screening), 30 min (kinetics)
MgCl2
UDPGA
8 . CBN-UGT1A8 (id=NPDI-ToTRjw)
In Vitro Enzyme Kinetics Experiment
Metabolism was detected.
- UGT1A8
Recombinant expression system
Baculovirus-insect cells
Cytochrome B5 No
Table 2
Results
Table 2
| Sample | Compound measured | Value | Measurement | Study sequence | Additional information | N replicates |
|---|
Experimental Conditions
Metabolite formation study
5 μg
In-house preparation
30 μL
90 min (screening), 30 min (kinetics)
MgCl2