Green Tea (Camellia sinensis) 19124972
Inhibitory effects of various beverages on ritodrine sulfation by recombinant human sulfotransferase isoforms SULT1A1 and SULT1A3 CSV JSON

PMID:16078151

16078151

1 . Green tea inhibition of SULT1A3 using dopamine as the substrate (id=NPDI-_Zkjzg)

In Vitro Enzyme Inhibition Experiment

Inhibition was detected.  Cutoff used — Not available

dopamine 1337860

green tea leaf

dopamine sulfate

  • SULT1A3

Recombinant expression system E.coli
Cytochrome B5 No

Results

The above results were observed at concentrations of 5 and 10%. At green tea 1%, dopamin sulfation was significantly inhibited by approximately 55% (estimated from Fig. 4B; P < 0.01).

Sample Compound measured Value Measurement Study sequence Additional information N replicates

Experimental Conditions

In-house preparation

50 µL

10 min (pH 6.8)

PAPS

1 µM

1, 5, and 10%

2 . Green tea inhibition of SULT1A1 using 4-nitrophenyl as the substrate (id=NPDI-mLlCzw)

In Vitro Enzyme Inhibition Experiment

Inhibition was detected.  Cutoff used — Not available

p-nitrophenol

green tea leaf

4-nitrophenyl sulfate

  • SULT1A1

Recombinant expression system E.coli
Cytochrome B5 No

Results

The above results were observed at concentrations of 5 and 10%. At green tea 1%, p-nitrophenol sulfation was significantly inhibited by approximately 90% (estimated from Fig. 4A; P < 0.01).

Sample Compound measured Value Measurement Study sequence Additional information N replicates

Experimental Conditions

In-house preparation

50 µL

10 min (pH 6.8)

PAPS

1 µM

1, 5, and 10%

3 . green tea inhibition of SULT1A3 using ritodrine as the substrate (id=NPDI-L5RmbA)

In Vitro Enzyme Inhibition Experiment

Inhibition was detected.  Cutoff used — Not available

ritodrine 1313200

green tea leaf

ritodrine sulfate ester

  • SULT1A3

Recombinant expression system E.coli
Cytochrome B5 No

Results

The above result was observed at 10% of green tea. At green tea 1 and 5%, ritodrine sulfation was significantly inhibited by approximately 25% (estimated from Fig. 2B) and > 95% (P < 0.01 for both), respectively.

Sample Compound measured Value Measurement Study sequence Additional information N replicates

Experimental Conditions

In-house preparation

50 µL

10 min (pH 6.8)

PAPS

3000 µM

1, 5, and 10%

4 . green tea inhibition of SULT1A1 using ritodrine as the substrate (id=NPDI-OQFZmw)

In Vitro Enzyme Inhibition Experiment

Inhibition was detected.  Cutoff used — Not available

ritodrine 1313200

green tea leaf

ritodrine sulfate ester

  • SULT1A1

Recombinant expression system E.coli
Cytochrome B5 No

Results

Sample Compound measured Value Measurement Study sequence Additional information N replicates

Experimental Conditions

In-house preparation

50 µL

10 min (pH 6.8)

PAPS

3000 µM

1, 5, and 10%