Green tea (Camellia sinensis)
Inhibitory effects of various beverages on ritodrine sulfation by recombinant human sulfotransferase isoforms SULT1A1 and SULT1A3
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1 . Green tea inhibition of SULT1A3 using dopamine as the substrate (id=NPDI-_Zkjzg)
In Vitro Enzyme Inhibition Experiment
Inhibition was detected. Cutoff used — Not available
- SULT1A3
Recombinant expression system
E.coli
Cytochrome B5 No
Results
The above results were observed at concentrations of 5 and 10%. At green tea 1%, dopamin sulfation was significantly inhibited by approximately 55% (estimated from Fig. 4B; P < 0.01).
| Sample | Compound measured | Value | Measurement | Study sequence | Additional information | N replicates |
|---|
Experimental Conditions
In-house preparation
50 µL
10 min (pH 6.8)
PAPS
1 µM
1, 5, and 10%
2 . Green tea inhibition of SULT1A1 using 4-nitrophenyl as the substrate (id=NPDI-mLlCzw)
In Vitro Enzyme Inhibition Experiment
Inhibition was detected. Cutoff used — Not available
- SULT1A1
Recombinant expression system
E.coli
Cytochrome B5 No
Results
The above results were observed at concentrations of 5 and 10%. At green tea 1%, p-nitrophenol sulfation was significantly inhibited by approximately 90% (estimated from Fig. 4A; P < 0.01).
| Sample | Compound measured | Value | Measurement | Study sequence | Additional information | N replicates |
|---|
Experimental Conditions
In-house preparation
50 µL
10 min (pH 6.8)
PAPS
1 µM
1, 5, and 10%
3 . green tea inhibition of SULT1A1 using ritodrine as the substrate (id=NPDI-OQFZmw)
In Vitro Enzyme Inhibition Experiment
Inhibition was detected. Cutoff used — Not available
- SULT1A1
Recombinant expression system
E.coli
Cytochrome B5 No
Results
| Sample | Compound measured | Value | Measurement | Study sequence | Additional information | N replicates |
|---|
Experimental Conditions
In-house preparation
50 µL
10 min (pH 6.8)
PAPS
3000 µM
1, 5, and 10%
4 . green tea inhibition of SULT1A3 using ritodrine as the substrate (id=NPDI-L5RmbA)
In Vitro Enzyme Inhibition Experiment
Inhibition was detected. Cutoff used — Not available
- SULT1A3
Recombinant expression system
E.coli
Cytochrome B5 No
Results
The above result was observed at 10% of green tea. At green tea 1 and 5%, ritodrine sulfation was significantly inhibited by approximately 25% (estimated from Fig. 2B) and > 95% (P < 0.01 for both), respectively.
| Sample | Compound measured | Value | Measurement | Study sequence | Additional information | N replicates |
|---|
Experimental Conditions
In-house preparation
50 µL
10 min (pH 6.8)
PAPS
3000 µM
1, 5, and 10%