Green Tea (Camellia sinensis) 19124972
Green tea ingestion greatly reduces plasma concentrations of nadolol in healthy subjects. CSV JSON

This study aimed to evaluate the effects of green tea on the pharmacokinetics and pharmacodynamics of the β-blocker nadolol. The study includes a trasport experiment using cells stably expressing the organic anion–transporting polypeptides OATP1A2 and OATP2B1.

PMID:24419562

24419562

1 . OATP1A2 in vitro inhibition (id=NPDI-wYUfKA)

In Vitro Transporter Inhibition Experiment

Inhibition was detected.  Cutoff used — not clearly specified

nadolol 1313200

  • OATP1A2 (SLCO1A2)

Transfected / siRNA knock-out / injected cells HEK293 transfected cells

Results

Sample Compound measured Measurement Value Study sequence Additional information N replicates

Experimental Conditions

Cell Culture Conditions

poly-D-lysine hydrobromide-coated 12-well plates

2

Assay Conditions

5 min

37 deg C

7.3

100 µM

1, 10, 100, and 1000 µM

not specified

2 . OATP1A2 inhibition with green tea (id=NPDI-lih1tg)

In Vitro Transporter Inhibition Experiment

Inhibition was detected.  Cutoff used — Not provided

nadolol 1313200

green tea leaf

  • OATP1A2 (SLCO1A2)

Transfected / siRNA knock-out / injected cells HEK293 transfected cells

Results

Sample Compound measured Measurement Value Study sequence Additional information N replicates

Experimental Conditions

Cell Culture Conditions

poly-D-lysine hydrobromide-coated 12-well plates

2

Assay Conditions

5 min

37 deg C

7.3

100 µM

0.1 - 10% (10:100 dilution of green tea)

commercial green tea beverage (Healthya; Kao, Tokyo, Japan)

1 . Green tea interaction with nadolol (id=NPDI-i6A3tw)

In Vivo Interaction Study

Increased systemic exposure was detected.

nadolol 1313200

green tea leaf

  • OATP1A2 (SLCO1A2)

This study suggests that green tea reduces plasma concentrations of nadolol possibly in part by inhibition of OATP1A2-mediated uptake of nadolol in the intestine.

Results

For AUC change, P < 0.001 (currently no such option in the build).

Sample Compound measured Measurement Value Study sequence Additional information N replicates

Experimental Conditions

Fixed-sequence

Population

Healthy volunteers

Nonsmokers

Asian

10

Ten Japanese subjects including eight males and two females; age: 20–30 years; body mass index: 18.3–23.9 kg/m 2

Pharmacokinetic (PK) Sampling Information

predose (0 h) and at 0.5, 1, 2, 3, 4, 6, 8, 24, and 48 h after nadolol administration

Drug or Natural Product Administration

Object Administration — nadolol

Oral

not provided

30 mg

single dose

Precipitant Administration — green tea leaf

Oral

liquid

700 mL

once daily

14 days

Natural Product Characteristics

commercial green tea beverage (Healthya; Kao, Tokyo, Japan)

2013

Pharmacodynamics (PD) & Adverse Events

As PD tests, PR, SBP, and DBP were recorded after 10 min rest in a sitting position at predose and at 0.5, 1, 2, 3, 4, 8, 24, and 48 h after nadolol administration using an automatic blood pressure monitor.

Cardiac disorders 35200000

In the water phase, nadolol lowered PR, SBP, and DBP, with maximum decreases of 20, 12, and 11%, respectively, based on arithmetic means. Coadministration of green tea tended to reduce the PD responses to nadolol in all parameters. In particular, green tea significantly suppressed the SBP-lowering effect of nadolol.